1. Enzyme preparation should be included in formula cost calculation

Bioengineered microbial phytase can degrade phytate, release available phosphorus, calcium, energy and protein, and release phosphorus, calcium and other nutrients in a linear increase at the recommended level. When the phytase addition level exceeds the addition amount of 500 FTU/kg, the release of nutrients continues to increase, but the amount of phytate released tends to decrease.

Therefore, the addition of phytase at the super recommended level is economically uneconomical. Β-glucanase and pentosanase are effective in degrading β-glucan and pentosan contained in certain raw materials in feed. These two water-soluble non-starch polysaccharides are anti-nutritional factors. These anti-nutritional factors bind a large amount of water and increase the viscosity of the digestive tract fluid. Reduces the effects of nutrient substrates and endogenous enzymes in the digestive tract, resulting in a decrease in the effectiveness of nutrients. The addition of β-glucanase and pentosanase to the corn-soybean-type diet with less anti-nutritional factors has no obvious effect on the improvement of animal performance; it is added to the day of rye, barley and wheat. Among the grains and the diets containing more unconventional feed ingredients, the animal performance improvement effect is greater.

The same addition amount with the increase of unconventional feed content in the diet, the improvement effect is more obvious; the same diet with the increase of the amount of enzyme added, the improvement effect is more obvious, but the effect of improving the unit enzyme is reduced. Regardless of the feed ingredients, excessive addition of beta-glucanase and pentosanase is economically uneconomical. In summary, when formulating lower cost diets and calculating benefits, the enzyme preparation should be included in the formulation cost calculation.

2, should consider the factors affecting the activity of enzyme preparations

The enzyme preparation itself is a type of protein, and any factor affecting the protein affects the activity of the enzyme preparation. The activity of the enzyme preparation increases with increasing temperature, but when the temperature is high to some extent, the enzyme is denatured and loses activity. Generally, the suitable temperature of the enzyme activity is 30 to 45 ° C. When the temperature exceeds 60 ° C, the enzyme denatures and loses activity. pH also has an effect on enzyme activity, and the enzyme is more active in a certain pH range when other conditions are constant. The optimum pH of the general enzyme activity is close to neutral (6.5 to 8.0). However, there are exceptions, such as a suitable pH for pepsin of 1.5. Monoiodoacetic acid, ferricyanide and heavy metal ions can bind or react with the essential groups of the enzyme, thereby deactivating the enzyme. Therefore, in the feed production process, we must pay attention to the effects of temperature, acid and alkali, heavy metal ions and other factors on the enzyme preparation, in order to achieve better use of the enzyme preparation.

3, the purchase of enzyme preparations should consider the effective content and price

There are many kinds of enzyme preparations on the market. When purchasing enzyme preparations, users must choose enzyme preparations that can ensure both effective content and cheaper. They should not only consider the price is cheap, and the effective content is not considered.

4, the use of enzyme preparations should consider feeding objects

The application of enzyme preparations in single stomach animals was obvious, and the effect of herbivores was not obvious. Therefore, the addition of an enzyme preparation may not be considered in the herbivore feed.

5, should pay attention to the quality inspection of enzyme preparations

Many feed testing departments now check the effective amount of enzyme preparations. When the user purchases, the sample can be sent to the relevant department for inspection to ensure the quality of the purchased enzyme preparation is reliable.

Existing enzyme assays are not fully applicable to the determination of enzyme activity in feed enzyme preparations

The detection temperature and pH specified by the industrial enzyme activity detection method are not necessarily the ambient temperature and pH at which the enzyme in the animal functions. For example, the industrial xylanase activity detection temperature is set to 50 ° C and the pH is 5.3, while the animal body temperature is about 40 ° C, and the enzyme catalyzes in the body - the small intestine pH is about 6.0. Therefore, if the feed enzyme is detected by the current industrial enzyme assay, it may affect the lateral comparison of the enzyme activities of different brands of feed enzyme preparations. That is, the use of industrial enzyme detection method to detect products with large enzyme activity does not necessarily have a large enzyme activity in the digestive tract of livestock and poultry.